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Jawaharlal Nehru Tropical Botanic Garden and Research Institute

Dr. K K Sabu

Dr K Satheeshkumar

Dr. K K Sabu
Senior Scientist
Biotechnology & Bioinformatics Division
 +91-9895211299
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  • Dr. Sabu is educated in the field of Plant Sciences and has working experience in plant genetics, biotechnology and bioinformatics since 1995. He published several research articles in national/international journals. He has currently supervising five graduate students for their PhD programme and carrying out three externally funded research programmes.

    Major Research Activities

    • Analysis of genetic diversity and phylogeny in Calamus L. (Arecaceae)
    • Analysis of genetic variability, association mapping and microRNA diversity of wild cardamom populations
    • Estimation of genetic diversity in Centella asiatica and regulation of secondary metabolite production using miRNAs

     

     

     

  • Ongoing

      • Project Title: Community plant-pollinator interactions at the landscape level.
        Name of the PI & Co-PI(s): Dr. B. Sabulal, Dr. Mathew Dan, and Dr. K. K. Sabu (JNTBGRI); Mr. Deepak Mundkinajeddu (M/s Natural Remedies Pvt Ltd, Bangalore).
        Funding agency and amount sanctioned: Department of Biotechnology, Government of India. 36.31 Lakhs.
        Project period and date of commencement: 3 years from 14.05.2015.

     

      • Project Title: Identification of elite lines of Centella asiatica and Bacopa monnieri for commercially significant constituents for standardization of their extracts
        Name of the PI & Co-PI(s): Dr. A. K. Sreekala, Dr. C. Anilkumar and Dr. K. K. Sabu (JNTBGRI); Dr. Hema Somanathan (IISER, Trivandrum), Dr. Deepak Barua (IISER, Pune), Prof. V. V. Belavadi (GKVK, Bangalore)
        Funding agency and amount sanctioned: Department of Biotechnology, Government of India. 59.23 Lakhs.
        Project period and date of commencement: 3 years from 14.05.2015

     

      • Project Title: Analysis of genetic variability and bioprospecting of wild cardamom populations.
        Name of the PI & Co-PI(s): Dr. K. K. Sabu.
        Funding agency and amount sanctioned: KSCSTE Plan (In-house). 24.84 lakhs (fund received till 4th year).
        Project period and date of commencement: 5 year programme started in April 2012.

     

     

    Completed

      • Project Title: Assessment of Genetic Diversity and Identification of Gender Specific Markers of Important North-East and South Indian Rattan palms using SSR Analysis.
        Name of the PI & Co-PI(s): Dr. K.K. Sabu & Dr. A.S. Hemanthakumar.
        Funding agency and amount sanctioned: Department of Biotechnology, Government of India. 16.35 Lakhs.
        Project period and date of commencement: Nov 2011 to Nov 2014

     

      • Project Title: Analysis of genetic diversity in selected rattan palms (Calamus sp.) using microsatellite markers.
        Name of the PI & Co-PI(s): Dr. K.K. Sabu.
        Funding agency and amount sanctioned: Kerala Biotechnology Commission, KSCSTE, Fund received: 10.34 lakhs.
        Project period and date of commencement: April 2012 to March 2014.

     

     

  • Major Achievements

    • Organized a germplasm collection of Andrographis paniculata from South East Asia and analyzed intraspecific variations of the species.
    • Six new rice varieties were developed and registered under PROTECTION OF NEW PLANT VARIETIES ACT 2004 in Malaysia in association with other researchers.
    • Developed mapping population of rice and conducted molecular breeding.
    • Genome size of cardamom was determined for the first time.
    • Micro RNA and transcriptome sequencing cardamom was carried out for the first time.

     

  • Publications

    1. Lakshmi Priya, P. M. and K. Sabu. 2015. In Silico Analysis of Transcriptomes of Catharanthus roseus and Rauvolfia serpentina, two potent medicinal plants using a pipeline developed from publicly available tools. American Journal of Bioinformatics Research 5(2) (Accepted).
    2. Thomas, J. and K. Sabu. 2015. Development of a New Pipeline for Identification and Characterization of Micro RNAs from Plants. International Journal of Computational Biology 4(2) (Accepted).
    3. Alireza Valdiani, Daryush Talei, Surrinder K. Lattoo, Jacqueline Batley, Rodomiro Ortiz, Mohd Yusop, Rafii, Mahmood Maziah, K. Sabu, Rambod Abiri, Suchirat Sakuanrungsirikul, Soon Guan Tan. 2015. Biotechnological and Conventional Advances of Plant Breeding in Andrographis paniculata: A Promising Breeding Platform for Autogamous Medicinal Plants. Critical Reviews in Biotechnology (Accepted).
    4. Princy, P. S., A. Gangaprasad and K. Sabu. 2015. In Vitro Propagation and Evaluation of Genetic Fidelity of Aegle marmelos (L.) Correa, a Highly Sought After Sacred Medicinal Tree. Phytomorphology 65 (1&2): 1-10.
    5. F., N. Anjali, A. Gangaprasad, K. K. Sabu. 2015. High quality RNA extraction from small cardamom tissues rich in polysaccharides and polyphenols. Analytical Biochemistry 485: 25-27. doi:10.1016/j.ab.2015.05.017
    6. Anjali, N., Sowmya S. Dharan, F. Nadiya and K. Sabu. 2015. Development of EST-SSR markers to assess genetic diversity in Elettaria cardamomum Maton. International Journal of Applied Sciences and Biotechnology, Vol 3(2): 188-192. doi: 10.3126/ijasbt.v3i2.12380
    7. Abdul Rahim H., Lim L.S., Sabu K.K, Saad A, Md. Atiqur Rahman B, Azhar M and Wickneswari R. 2012. Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice. Genetics and Molecular Research 11 (3): 3277-3289 (Sept 2012).
    8. Wickneswari R., M.A.R. Bhuiyan, K. Sabu, L.S. Lim, M.J. Thomson, N.M. Kairudin and Abdullah M.Z. 2012. Identification and Validation of Quantitative Trait Loci for Agronomic Traits in Advanced Backcross Breeding Lines Derived from Oryza rufipogon × Oryza sativa Cultivar MR219. Plant Mol Biol Rep 30(4): 929-939, DOI: 10.1007/s11105-011-0404-4 (Published on 17 Jan 2012)
    9. Ngu M.S., Sabu K.K., Lim L.S., Abdullah M.Z, Wickneswari R. 2010. Genetic structure of Oryza rufipogon natural populations in Malaysia: Implications for conservation and genetic introgression of cultivated rice. Tropical Plant Biology 3(4): 227-239.
    10. Sabu K.K., Abdullah M.Z., Lim L.S. and Wickneswari R. 2009. Analysis of heritability and environmental variances in a rice cross. Agronomy Research 7: 97-102.
    11. Sabu K.K., Abdullah M.Z., Lim L.S and Wickneswari R. 2006. Development and evaluation of advanced backcross families of rice for agronomically important traits. Communications in Biometry and Crop Science 1(2): 111-123
    12. Sabu, K.K., Padmesh and S. Seeni. 2001. Estimation of active principle content and isozymes of Andrographis paniculata, an important medicinal plant of India. Journal of Medicinal & Aromatic Plant Sciences 23: 637-647.
    13. Jacob, J.P., K.K. Sabu and T.K. Abraham. 2000. Genetic variability of mutant strains of Pleurotus citrinopileatus based on isozyme electrophoresis. Mushroom Research 9: 79-84.
    14. Padmesh, P., K. Sabu, S. Seeni and P. Pushpangadan. 1998. The use of RAPD in detecting genetic variability in Andrographis paniculata Nees: a potent hepatoprotective drug. Current Science 76: 833-835.
    15. Seeni, S., K. Sabu and P. Padmesh. 1998. Variable-Invariably: An introduction to intraspecific variations in medicinal plants. Amruth 2: 3-8.

     

    Books/Book Chapters/Thesis

    Sabu, K. K., Nadiya. F., and N. Anjali. 2016. miRNA isolation from plants rich in polysaccharides and polyphenols. In: Micro RNA Profiling: Methods and Protocols. Sweta Rani (Ed). Springer, New York. (Accepted)

    Harinarayanan, P. & Sabu, K. K., (Eds.). 2009. Proceedings of National Seminar on Coastal Ecosystem Management & Evaluation Workshop of South Indian ENVIS Centres.  ENVIS Centre, Kerala State Council for Science, Technology, & Environment, Thiruvananthapuram. 126 pages.

    Sabu, K.K., N.S. Pradeep and P. Padmesh. 2003. Application of molecular markers for the analysis of genetic diversity and systematics of fungi. In Frontiers of Fungal Diversity in India (Prof. Kamal Festschrift) Ed. by G. P. Rao, C. Manoharachari, D. J. Bhat. Published by: International Book Distributing (Lucknow), xxiv, 906p. ISBN: 8185860920.

    KK Sabu. 2002. Intraspecific variations in Andrographis paniculata Nees (PhD Thesis). Kerala University, Thiruvananthapuram, India

    Seeni, S. and K.K. Sabu. 1998. Conservation and economic utilization of plant genetic resources through Biotechnological means. In: Conservation and Economic Evaluation of Biodiversity. Vol. I. pp. 239-249.  Oxford University press, New Delhi.

     

    Conference Papers  

    Binoy Kurian, Vishnu V. Nair, and K.K. Sabu. 2015. Paucity of genetic diversity in an important non-wood forest produce: Calamus brandisii Becc. – A dwindling species? Proceedings of the 27th Kerala Science Congress held at Alappuzha during 27th – 29th January 2015.

    Sowmya S Dharan, Anjali N, Nadiya F and K K Sabu. 2014. Variation in lignin content and genetic diversity in Elettaria cardamomum Maton. Proc. of International Symposium on Plantation Crops – PLACROSYM XXI” from 10-12 December, 2014 at Kozhikkode.

    Krishna R. Nair, Gangaprasad A. and K. K. Sabu. 2014. An efficient plant regeneration from nodal explant of Somalata [Sarcostemma acidum (roxb.) Voight] and assessment of genetic uniformity using ISSR markers. Proceedings of the 26th Kerala Science Congress held at Wayanad during 28th – 31st January 2014, pp. 316 (10-90).

    Anjali N, Nadiya F and K K Sabu. Genome size estimation using flow cytometry in Elettaria cardamomum Maton. Proc. 16th All India Conference of Cytology and Genetics and National Symposium on ‘Gene, Environment and Health’. October 22-24, 2013. Dept of Botany, Kerala University, Thiruvananthapuram. Page 83-84.

    Binoy Kurian, Vanila Varghese, Vishnu V. Nair, Hemanth Kumar A.S. and K.K. Sabu. 2013. Analysis of genetic diversity in an economic rattan palm, Calamus thwaitesii Becc. using SSR and ISSR markers. Proc. National Seminar on Tree Biotechnology 2013 organised at the Institute of Forest Genetics and Tree Breeding, Coimbatore from 23-24 September 2013. (Eds.) Madhumita Dasgupta, Rekha R. Warrier and R. Yasodha. Page 226-228.

    Ashitha J Prakash, Anija T Anto, Anjali, N and K K Sabu. 2013. Genetic diversity among cardamom germplasm accessions assessed by SSR and ISSR analysis. Proceedings of the 25th Kerala Science Congress held at Technopark at Thiruvananthapuram during 29th January to 1st February 2013, pp. 263-265 (03-27).

    Sabu, K.K., M. Kamarudeenkunju, N. Anjali, J.P. Ashitha, A.S. Hemanthkumar and P.N. Krishnan. 2012. Analysis of genetic diversity and identification of gender-specific ISSR markers in Calamus thwaitesii Becc. International Conference on Advances in Biological Sciences (ICABS). March 15-17, 2012. Dept of Biotechnology and Microbiology and Inter University Centre for Biosciences, Kannur University, Kannur.

    Li Sze Lim; K. K. Sabu, Mee Siing Ngu; Md Atiqur Rahman Bhuiyan; Abdullah Mohammed Zain; Michael J. Thomson; Wickneswari Ratnam. 2009. Identification of quantitative trait loci for agronomic traits in an advanced backcross population between Oryza rufipogon Griff. and the Malaysian Oryza sativa l. cultivar MR219 and fine mapping. Proc. 8th Malaysia Congress on Genetics, 4-6 August 2009, Genting Highlands, Malaysia

    Lim, L. S., Ngu, M. S., K. K. Sabu, Bhuiyan, M. A. R., Zain, A. M., Thomson, M. J., Wickneswari, R. 2009. Identification of quantitative trait loci for agronomic traits in an advanced backcross population between Oryza rufipogon Griff. and the Malaysian Oryza sativa L. cultivar MR219 and fine mapping. Proceedings of 8th Malaysia Congress on Genetics, 4-6 August 2009, Genting Highlands, Malaysia

    Kokkal, K., P. Harinarayanan and K.K.Sabu. 2008. Wetlands of Kerala. In: Proceedings of Taal 2007: 12th World Lake Conference held at Jaipur. Pg.1889 to 1893

    Ngu, M.S., Sabu K.K., Lim L.S. Abdullah M.Z., and Wickneswari R. 2007. Genetic structure of Oryza rufipogon Griff. in Malaysia. 6th Asian Crop Science Association Conference and 2nd International Conference on Rice for the Future. November 5-9, 2007. Queen Sirikit National Convention Center in Bangkok, Thailand.

    Sabu K.K., Abdullah M.Z., Lim L.S. and Wickneswari R. 2005. Heritability and genetic variance estimates for yield and yield related traits in Oryza rufipogon/O. sativa F1 and BC2F2 families. 5th International Rice Genetics Congress held at International Rice Research Institute (IRRI), Manila in November 2005.

    Lim, L.S., K.K. Sabu, R. Wickneswari and M.Z. Abdullah. 2005. Identification of QTLs for tillering ability in an inter-specific rice cross.  Plant & Animal Genomes XIII Conference, January 15-19, 2005. Town & Country Convention Center, San Diego, USA.

    Sabu, K.K. 2005. Using SAS Procedures to Estimate Genetic Variances and Heritability in a Rice Cross. Proc. of 19th Annual SAS User’s Malaysia (SUM) Forum, Kuala Lumpur Convention Centre. 21 Sept 2005.

    Geetha, B.S., K.K. Sabu and S. Seeni. 2003. Genetic variation in south Indian populations of Phyllanthus amarus Schum. & Thonn. (Euphorbiaceae) assessed using isozymes. Proceedings of the 15th Kerala Science Congress. 29-31 January 2003, Thiruvananthapuram, pp. 196-201.

    Nadarajah, K., Song, B.K., Sabu, K.K. and  Ratnam, W. 2003. The Use of microsatelite markers in physical and genetic mapping of Malaysian rice variety. International Conference for Bioinformatics, Penang Malaysia. Pg 111 (D003).

    Lim L.S, Sabu K.K., Wickneswari R. and Abdullah M.Z. 2003. QTL identification of yield-related traits in a Malaysian rice cross. Int Rice Conference, 13-17 Oct 2003, Alor Setar, Malaysia

    Wickneswari R., Lim L.S., Sabu K.K. and Abdullah M.Z. 2003. Improvement of grain yield using AB-QTL strategy in a Malaysian rice variety. Oral paper presented at the 14th National Biotechnology Seminar, 11-13 December 2003, Penang, Malaysia.

    Wickneswari R., Sabu K.K., Lim L.S and Abdullah M.Z. 2003 Improvement of grain yield using advanced backcross QTL strategy in a Malaysian rice variety. International Genetics Congress, 6-11 Jul 2003, Melbourne, Australia.

    Sabu, K.K. and Seeni, S. 2003. In situ and ex situ genetic diversity of Andrographis paniculata Nees populations from India. Int Genetics Congress 2003, 6-11 Jul 2003, Melbourne, Australia

    Sabu, K.K. and S Seeni. 2001. PlantPack: a new software for analysis of diversity within Andrographis paniculata, an important medicinal plant, In: Proc. Kerala Science Congress (13th), January 2001, Thrissur  pp. 688-690.

    Sabu, K.K., S Seeni and G Sreekantan Nair. 2001. The application of Bioinformatics in bioprospecting of medicinal plant resources of Kerala. In: Proc. 13th Kerala Science Congress, January 2001, Thrissur. Theme Session: information Technology for Development – A Millennium Perspective, pp. 46-50.

    Padmesh P., K.K. Sabu and S. Seeni. 1999. Analysis of genetic variations in Andrographis paniculata using isozyme and random amplified polymorphic DNA markers. Proc 11th  Kerala Science Congress, 27 Feb.-1 Mar. 1999, Kasaragod- (India), pp. 95-100

    Sabu, K.K. and S. Seeni. 1999. Development of a computer package for estimation of various plant molecules. Symposium on Current Trends in Biology, Indian Institute of Science, Bangalore. 27-28 Dec, 1999.

    Sabu, K.K. and S. Seeni. 1998. Patterns of allozyme diversity in natural populations of Andrographis paniculata Nees. National Conference on Recent Trends in Spices and Medicinal Plant Research, Bose Institute, India, 2-4 April, 1998

    Sabu, K.K. and S. Seeni. 1996. Biochemical characterization of intraspecific variations in Andrographis paniculata Nees. International Symposium on Perspectives in Biochemistry and Molecular Biology, Indian Institute of Science, Bangalore. 20-23 Nov, 1996.

     

    Other Publications

    • ENVIS Newsletter (Vol.1, Nos 1-4; Vol.2, Nos.1-2) (Member, Editorial Board)
    • A handbook (in Malayalam) on water-related diseases prevalent in Kerala (Member, Project Team)
    • Kalavastha Vyathianum (in Malayalam), a book on climate change (Member, Project Team)
    • Proceedings of the ENVIS workshop held at Munnar on 2-3 April 2009 (Member, Editorial Board)

     

  • Anjali

    Ms.Anjali.N – Senior Research Fellow
    Research Topic: Genetic structure and differentiation of wild and domesticated populations of cardamom (Elettaria cardamomum (L.) Maton).
    Research Topic Summary: As in the case of other crop plants, wild cardamom plants may possess genes that can be effectively used for the crop improvement programmes. Extensive studies of miRNAs have been done in many plant species including crop plants. However, such studies in wild relatives of the crop plants are lacking which are very important owing to the potential existence untapped genetic diversity and disease resistance characteristics in these plants. Ms. Anjali is working on the identification and characterization of miRNAs from wild cardamom.
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    Nadiya

    Ms.Nadiya.F – Senior Research Fellow
    Research Topic: Identification and characterization of miRNAs and deferentially expressed genes in Elettaria cardamomum Maton.
    Research Topic Summary: Identifying miRNA targets will improve our understanding of the miRNA-mediated mechanisms of plant defense response. Though studies on miRNAs have been done in many plant species including crop plants, such studies in wild relatives of the crop plants are lacking which are very important owing to the potential existence untapped genetic diversity and disease resistance characteristics in these plants. In the proposed study, it is aimed to determine which cellular miRNA plays a role in the cardamom mosaic virus (CdMV) infection through comprehensive miRNA profiling in wild cardamom.
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    Mr. Binoy Kurian   Junior Research Fellow
    Research Topic: Development of molecular markers for analysis of genetic diversity and phylogeny in Calamus L. (Arecaceae)
    Research Topic Summary: Populations of Calamus (rattan palms) are scattered in small groups and most of the plants are destroyed in the natural stands due to rapid deforestation. Unscientific harvesting of canes from matured female plants often destroys the plants and hampers seed propagation. Thus, proper female:male ratio is not maintained in the wild. It is therefore very much essential to identify plants at an early generation to check the loss of seed bearing female plants. Identification of DNA markers associated with gender will provide guidance in early generation selection of plants and help in managing Calamus propagation. Moreover, development of gender -specific DNA markers will help in selecting male and female plants from natural stands and will thus aid in designing an appropriate rattan breeding strategy along with development of propagating materials.
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    Sakthipriya

    Ms.Sakthipriya.M  –  Junior Research Fellow
    Research Topic: Genetic association mapping for major quantitative traits in Elettaria cardamomum Maton.
    Research Topic Summary: Complex traits are controlled by multiple genes as well as influenced by environmental factors. Understanding the genetic basis of complex traits or locating allelic regions associated with these traits can be useful in genetic studies and breeding to manipulate the important quantitative traits. Recently, association mapping (AM) has become an important method for dissecting the genetic basis of complex traits in plants. Ms. Sakthipriya will apply association mapping to identify genes associated with complex traits in cardamom which would leads to map the possible QTLs and also provide the knowledge about the genome organization of the cardamom.
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    Gouri

    Ms.Gouripriya Ranjith – Junior Research Fellow
    Research Topic: Regulation of alkaloid biosynthesis by microRNA in Centella asiatica.
    Research Topic Summary: Plant secondary metabolites are economically important as drugs, fragrances, pigments, food additives and pesticides. Recent advances in combined molecular biology and enzymology through post-transcriptional gene silencing (PTGS) of medicinal plants suggest that this system is a viable source of important secondary metabolites. Gouri aims toidentify the possible relation between alkaloid biosynthesis and microRNA (miRNA) regulation in Centella asiatica, which might prove to be useful for the studies towards formation of important biomedical molecules by the medicinal plant, which in turn can provide novel and rapid applications ranging from medicinal plant cultivation to herbal therapeutics.
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    Jinu

    Ms.Jinu Thomas – Junior Research Fellow
    Research Topic Summary: The project aims to identify elite genotypes of Centella asiatica and Bacopa monnieri, two important medicinal plants. Jinu will be developing microsatellite markers through bioinformatics approach and assist related project activities.
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    Shefeek

    Mr.Shefeek.S – Project Assistant
    Project Details:The project aims to analyse plant-pollinator interactions using molecular markers.
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    Athira

    Ms.Athira V Nair – Technical Assistant
    Project Details:The project aims to collect wild populations and disease escapes of cardamom in addition to landraces and traditional cultivars and to estimate genetic diversity in the samples.
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